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Fig. 5. Averages from seven separate experiments of C2C12 cells grown in HS-2; HS-2+GAL-M; HS-2+COS-1; FCS-20; FCS-20+GAL-M, or FCS-20+COS-1. Results show percentage of nuclei present in myotubes. Asterisk (*) indicates a significant difference from FCS-20 or FCS-20+COS-1 tested by one-way ANOVA and post-hoc multiple paired comparison (P<0.05). Higher percentages of nuclei in myotubes were observed in galectin-1-treated cultures.