Fig. 8. Giemsa stained primary myoblast cultures grown for 5-6 days in HS-2 (A); HS-2+GAL-M (B); HS-2+COS-1 (C); FCS-20 (D); FCS-20+GAL-M (E); or FCS-20+COS-1 (F). Three different cell types, myoblasts, fibroblasts and myotubes, are present in these populations. Fibroblasts (A, white arrow), myotubes (B, white arrow) and myoblasts (C, white arrow) are evident in these cultures. Myotubes in GAL-M-treated cultures appeared more numerous and larger than in other media types (arrows, B,E). Bars, 50 µm.