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Fig. 5. Mutation of the leucine-rich NES impairs export of NT-GFP. (A) Leucine residues were mutated to valines at the two positions shown. An immunoblot of nuclei and cytoplasms isolated from oocytes expressing GFP, GFP-NT and GFP-NTNES– in the presence and absence of CHX (50 µg/ml) is shown. (B) Translocation of anti-myc IgG from the cytoplasm to the nucleus and from the nucleus to the cytoplasm in oocytes expressing GFP, GFP-NT and GFP-NTNES– is shown. (C) The green fluorescence (FL) and DAPI images are of HeLa cells expressing GFP-NT and GFP-NTNES–.