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Fig. 6. (A) Characterisation of the anti-Sso1p and anti-Sso2p antisera. Cell lysates from wild-type (W303-1A), sso1{Delta} (H403) and sso2{Delta} (H404) cells (10 µg of total protein) and 15 ng of purified Sso1-GST and Sso2-GST fusion proteins were analysed by western blotting using the specific antisera against Sso1p or Sso2p. Molecular weight markers are shown on the right. (B) Expression of wild-type and mutant Sso1p and Sso2p at permissive and restrictive temperatures. Wild-type (W303-1A), sso1-1 sso2{Delta} (H1239), and sso1{Delta} sso2-1 (H603) cells grown at 24°C were shifted to either 24°C or 38°C. Samples were taken at 0, 15, 30, 60 and 120 minutes after the temperature shift. Cell lysates were prepared and 20 µg of total protein was analysed by western blotting using the specific antisera against Sso1p or Sso2p.