Fig. 7. Suppression of the temperature-sensitive sso2-1 mutation by overexpression of other genes that are involved in secretion. H902 cells (sso1 sso2-1) containing different SEC genes on high copy number plasmids from the genomic pHR81 library were grown at 24°C, and then replicated to plates that were incubated at different temperatures, as shown in the figure. The vector control is pHR81 (Nehlin et al., 1989). Plasmid pMO9 carrying the SSO2 gene on a single copy plasmid was included as a control (SSO2 CEN in the figure).