Fig. 5. Nuclear protein import and export are normal in sar1-1, sec31-1 and pmm1-1. Wild-type, sar1-1, sec31-1 and pmm1-1 cells with integrated GFP-Pap1p under the medium strength nmt1 promoter were incubated at 25°C until mid-log phase, then shifted to 36°C for four hours. Cells were photographed either without addition of hydrogen peroxide, showing that GFP-Pap1p is exported from the nucleus (A,C,E,G) or after exposure to 0.8 mM hydrogen peroxide for 15 minutes, showing that GFP-Pap1p is imported into the nucleus (B,D,F,H). Bar, 10 µm.