Fig. 3. Inactivation of RhoA inhibits CD82-induced morphological changes. Jurkat cells were electroporated with Lucifer Yellow (1 mg/ml) with or without 20 µg/ml C3 exoenzyme from C. botulinum. After two hours in culture, cells were tested for their response to CD82 stimulation, as described in the legend of Figure 1. (a) Jurkat cells electroporated with Lucifer Yellow alone. (b) Cells electroporated with both Lucifer Yellow and C3 exoenzyme. The data shown are representative of three independent experiments. Bar represents 10 µm.