Fig. 4. Expression of N17Rac1 or N17Cdc42 inhibits most of the CD82-induced morphological changes but different cytoskeletal rearrangements still occur. Jurkat cells were transfected with 3 µg GFP and 15 µg of empty vector (a), c-myc-tagged N17Rac1 (b,d) or N17Cdc42 (c,e) as described in Materials and Methods and sorted by flow cytometry by their GFP expression after 48 hours in culture. Cells were stimulated for two hours by culture on anti-CD82-coated plates and assayed for morphological changes as described in the legend of Fig. 1. The data shown are representative of three independent experiments. Bar represents 10 µm.