Fig. 2. ß₃-endonexin downregulates uPAR promoter activity. (A) CAT-assay. CHO cells were transiently co-transfected with the -398 bp fragment of the uPAR promoter (uPAR-CAT -398) (1 µg), a luciferase vector for normalization and various concentrations of expression vectors encoding the short or long form of ß₃-endonexin or the mock expression vector (GFP). Cell extracts normalized for luciferase activity were incubated with [¹⁴C]chloramphenicol, extracted with ethyl acetate, and subjected to thin-layer chromatography. The conversion of [¹⁴C]chloramphenicol to acetylated derivatives was quantified using BioRad GelDoc scanning software. (B) Histobars depict the results (mean±s.e.m.) of the CAT-assays performed as described in A with 3 µg of the expression vector. The data shown is representative of six independently performed experiments.