Fig. 3. ß₃-endonexin localizes to the cell nucleus of endothelial cells. (A) Confocal laser microscopy. HUVECs were transiently transfected with GFP control vector, GFP/En-S, GPF/En-S-K⁶²RKK or GFP/En-L and the cellular localization of GFP fluorescence was studied by confocal laser scanning microscopy. (B) Immunoblot. Endothelial cells were transiently transfected with GFP/En-S, GPF/En-S-K⁶²RKK, or GFP/En-L, and cytosolic and nuclear extracts were probed by immunoblotting with anti-ß₃-endonexin polyclonal antiserum.