JCS -- Burda et al. 115 (20): 3889 Figure IG2

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Fig. 2. The CPY sorting receptor Vps10p is mislocalized to the vacuolar membrane in ${Delta}$ vps30 and ${Delta}$ vps38 mutant strains. Wild-type (PBY34), ${Delta}$ vps30 (PBY38) and ${Delta}$ vps38 (PBY40) cells expressing Vps10-GFP were labeled in YPD with the fluorescent dye FM4-64 for 10 minutes at room temperature. The dye was washed away, the cells were chased for an additional 60 minutes in YPD and then put on ice. Localization of FM4-64 and Vps10-GFP were compared by fluorescence microscopy.