JCS -- Burda et al. 115 (20): 3889 Figure IG4

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Fig. 4. ${Delta}$ vps30 and ${Delta}$ vps38 mutant cells exhibit defects in PtdIns3P synthesis. Wild-type (SEY6210), ${Delta}$ vps30 (JCY300), ${Delta}$ vps38 (PBY58), and ${Delta}$ vps30 ${Delta}$ vps38 (PBY51) cells were grown in YNB to early log phase and labeled with myo-[2-³H] inositol at 26°C for 45 minutes. Cellular lipids were recovered, deacylated and separated by HPLC. Levels of deacylated products corresponding to the indicated phosphoinositides are shown. These data represent the means±s.e.m. of at least three independent experiments. PtdIns3P, phosphatidylinositol 3-phosphate; PtdIns(4)P, phosphatidylinositol 4-phosphate; PtdIns(3,5)P₂, phosphatidylinositol (3,5)-bisphosphate; PtdIns(4,5)P₂, phosphatidylinositol (4,5)-bisphosphate.