Fig. 3. Intracellular localization and expression of EGFP-WRN fragments. (A) HeLa cells were transfected with the vector alone (EGFP) (a,b), EGFP-WRN (949-1092) (c,d) and EGFP-WRN (54-946) (e,f) constructs, and analyzed as described in Fig. 1A,B. One representative picture of each construct is shown. (B) Protein expression of EGFP-WRN constructs shown in panel A. After transfection the cells were analyzed as described in Fig. 2D.