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Fig. 4. Intranuclear localization of EGFP-WRN fragments in different human cell lines. U-2 OS (left) and AG11395 cells (right) were transfected with EGFP-WRN (a-d), EGFP-WRN (1072-1432) (e-h) and EGFP-WRN (949-1092 +NLS) (i-l) constructs as indicated on the left. After transfection the localization of each construct was analyzed by confocal microscopy.