Fig. 6. The RQC domain deletion mutant of WRN localizes outside the nucleoli. (A) Schematic representation of the generation of the WRN853-1089 (I853L). Numbers correspond to amino acid sequences. (B) HeLa cells were transfected with EGFP-WRN853-1089 (I853L) and analyzed as described in Fig. 1A,B. One representative picture is shown.