Fig. 1. Characterization of anti-NCS-1 antibodies. Total homogenates (40 µg) from rat brain (R.B.) and human cerebellum (H.C.) were analyzed by western blots using polyclonal (A, 44162; diluted 1:1000) or monoclonal antibodies (C, 3D5; diluted 1:5000) raised against recombinant NCS-1. Parallel samples were immunostained with preimmune rabbit or mouse IgG (IgG in A and C) or with the anti-mouse IgG, omitting the primary monoclonal antibody (C, no Ab). In B, proteins from rat brain total homogenate (R.B.) were labeled with anti-NCS-1 (44162) with (+) or without (-) pre-adsorption with 1 µg of purified recombinant NCS-1. In C, the arrow indicates the position of NCS-1; the bands of 55 and 28 kDa correspond to rat Ig heavy and light chains, respectively, which were present in the brain extracts and were recognized by the secondary antibodies (no Ab).