Fig. 10. NCS-1 distribution in UTP-stimulated PC12 cells. The cells grown on petri dishes were incubated for 3 minutes at 37°C in KBR in the absence (CON) or presence of 300 µM UTP (UTP) or in KBR minus Ca²⁺ supplemented with 2 mM EGTA and UTP (UTP-Ca⁺⁺_OUT). The cells were homogenized and the and cytosolic (C) and membrane (M) fractions were obtained. Equal volumes of the fractions were analyzed by western blotting using anti-NCS-1 or anti-tubulin antibodies and the amount of NCS-1 in the cytosol or in the membrane were quantified. (A) Data are expressed as a percentage of total NCS-1 measured in the cytosolic and membrane fractions after no treatment (CON) or treatment with UTP or UTP minus Ca²⁺. The results represent the mean±s.e.m. (^*P<0.001 compared with control). (B) Immunoblots showing the relative membrane/cytosol distribution of NCS-1 and tubulin (TUB) in membranes and cytosol of control and stimulated cells in the presence or absence of Ca²⁺.