Fig. 12. PI4Kß distribution in NCS-1-overexpressing PC12 cells. Cells were electroporated with cDNAs coding for NCS-1 and GFP (GFP+NCS-1) or GFP alone (GFP). 48 hours after transfection, the cells were incubated for 3 minutes at 37°C in KBR in the absence or presence of 300 µM UTP (+UTP) and then homogenized. The cytosolic (C) and membrane (M) fractions were prepared and analyzed by western blotting using anti-PI4Kß or NCS-1 antibodies. In A, the amounts of PI4Kß in the membrane fractions were quantified as described in Fig. 11 and the results are expressed as a percentage of the control (GFP-transfected cells) and represent the mean±s.e.m. (n=6, ^*P<0.002 compared with GFP-transfected cells). (B) Immunoblots showing the distribution of NCS-1 in the membranes and cytosol of GFP and NCS-1+GFP-transfected cells.