Fig. 4. NCS-1 immunoreactivity in the Golgi area. Ultrathin frozen sections from rat brain cortices were labeled for NCS-1 (A) and TGN38 (C) or double-immunostained (D) for NCS-1 (6 nm gold particles, arrowheads) and TGN38 (12 nm gold particles, arrows). In B, a section immunolabeled with rabbit IgG (control) is shown. NCS-1 immunoreactivity is present in the area of the Golgi complex (gc). The anti-TGN38 antibody specifically immunostains tubular-like elements corresponding to the TGN (tgn, C). Some labeling for NCS-1 (D, arrowheads) is detectable on the tubular-like structures immunolabeled with the anti-TGN38 antibodies (D, arrows). Note the lack of labeling when non-immune rabbit IgGs are used for immunostaining (B). Bars, 200 nm.