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Fig. 8. Association of NCS-1 with PI4Kß. Triton X-100 rat brain (600 µg) or PC12 cell (1 mg) extracts were immunoprecipitated (IP) with antibodies against NCS-1, PI4Kß (PI4K) or non-immune rabbit IgG (IgG). The immunoprecipitated proteins were analyzed by western blotting using rabbit anti-PI4Kß IgG or polyclonal anti-NCS-1 antibodies.