Fig. 3. PAK4 induces loss of paxillin-associated focal complexes. MDCK cells were microinjected with a plasmid encoding either PAKGBD or PAK4wt and incubated for 3 hours in 0.2% FCS before stimulation for 3 hours with HGF. Cells were then fixed and stained for HA-tagged PAK4 (A,B) and paxillin (C,D). Arrows indicate the absence of paxillin-associated focal complexes in a PAKGBD-expressing cell (C) and the presence of paxillin-associated focal complexes in a cell expressing PAK4wt (D). Bar, 10 µm.