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Fig. 4. PAK4 is localised at the cell periphery. MDCK cells were microinjected with plasmids encoding PAK4{Delta}GBD or PAK4wt and incubated for 5.5 hours in 0.2% FCS (A,C) or 3 hours in 0.2% FCS prior to stimulation with HGF for 2.5 hours (B,D). (E) MDCK cells were microinjected with a plasmid encoding PAK4{Delta} and incubated for 4.5 hours in 0.2% FCS. Cells were then fixed and stained for HA-tagged PAK4 and F-actin. Bar, 10 µm. The arrow (F) indicates a PAK4{Delta}GBD-expressing cell; arrowheads (D) indicate PAK4wt localisation at the periphery.