Fig. 3. The two murine UNC-45 mRNAs are differentially expressed. Duplicate northern blots of total RNA from various adult mouse organs and from 12 day embryos were labeled with isoform-specific probes (SM, skeletal muscle; He, heart; Ut, uterus; In, large intestine; Ki, kidney; Sp, spleen; Lu, lung; Br, brain; Li, liver; Ov, ovary; Em, embryo). The GC UNC-45 isoform was detected in all adult organs examined, and the SM UNC-45 isoform was detected in organs consisting mainly of striated muscle. A small amount of GC UNC-45 mRNA was also present in striated muscle tissues. The minor SM UNC-45 band in the lung sample is of unknown origin. Labeling of 18 S RNA indicated comparable loading.