Fig. 8. Reduction of SM UNC-45 mRNA did not alter skeletal myosin expression in myotube cultures. A Commassie blue-stained 7.5% polyacrylamide gel shows equal amounts of total protein from myotube cultures treated for 8 days with none (0), a negative control (C), GC UNC-45 antisense (G) or SM UNC-45 (S) antisense oligonucleotide. Myosin heavy chain (MHC) (arrow) and molecular weight markers are indicated. The lower panel shows the results of immunoblotting with MF 20 antibody to specifically detect skeletal MHC in the samples above. Equal amounts of skeletal MHC were in all but the GC UNC-45 antisense-treated cultures, which contained more myoblasts and few large myotubes.