JCS -- Gauczynski et al. 115 (21): 4025 Figure IG6

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Fig. 6. Anti-FLAG-antibody purification of FLAG-tagged prion proteins from BHK cells. (A and B) Total cell extracts from transfected BHK cells expressing huPrP227FLAG (A) or boPrP239FLAG (B) were analyzed by western blotting employing the mAb anti-PrP 3B5. In order to purify FLAG-tagged PrP, crude lysates (A and B, lanes 1) were bound to anti-FLAG M2 affinity gel (A, B, lanes 2) and eluted by competition with FLAG peptides (A and B, lanes 3). The arrows indicate the heavy and the light chain of the mAb anti-FLAG M2 (A and B, lanes 2). (C) The silver-stained gel shows purified glycosylated huPrP227FLAG and boPrP239FLAG.