Fig. 1. Pre-sequences of fusion proteins of BiP (A) and AtpC (B) from Phaeodactylum tricornutum fused to GFP, and various fusions in which part of the AtpC pre-sequence was deleted. The signal sequences and the N-terminus of GFP are in small letters, while the transit peptide domains are shown in capital letters. The bars below represent the signal sequences (black), transit peptides (grey), N-termini of BiP and AtpC (white) and GFP (stripes). Arrows mark probable cleavage sites for signal sequences. Cleavage sites were determined by N-terminal sequencing, as described in the Results.