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Fig. 4. Accumulation of GFP in the nuclear membrane (A) and on the plastid (B) surface of P. tricornutum expressing AtpC5-DDEL. Localization was demonstrated by immuno-electron microscopy using an antiserum against GFP (for details, see Materials and Methods). Arrows indicate areas that exhibit high GFP accumulation. P, plastid; N, nucleus; M, mitochondrion.