Fig. 4. 3D reconstruction of PLP genomic in situ hybridization merged with SC35 immunostaining in oligodendrocytes. The PLP gene (green) co-localizes with SC35 immunostaining (red) to appear as yellow at the periphery of nuclear domains enriched in SC35 in oligodendrocytes. Oligodendrocytes are shown as a 3D reconstruction imaged in the XY plane (A) and rotated 90 degrees to the XZ view (B). In progenitors shown as a 3D reconstruction, the PLP gene does not co-localize with SC35 imaged in the XY plane (C) or when rotated 90 degrees to the XZ view (D). The blue line outlines the nuclear periphery. Bar, 5 µm.