Fig. 2. Bgs1p localizes to the growing areas, to one or both poles, and to the contractile ring and septum. (A) Calcofluor white staining and GFP-Bgs1p localization along the mitotic cell cycle. (B) Detail of Calcofluor white staining and GFP-Bgs1p localization to the contractile ring and along the plasma membrane during septum formation. Early-logarithmic phase cells (GFP-bgs1⁺ bgs1), grown on YES liquid medium at 28°C, were visualized for GFP and Calcofluor white staining. Calcofluor white was added at 50 µg/ml following immediate examination of the cells. Cells representative of each cell cycle step were selected and aligned to show a cell cycle progression.