Fig. 3. Bgs1p localization during septation depends on the medial ring formation and localization, and on proteins that form the septation initiation network. Calcofluor white staining and GFP-Bgs1p localization in different cdc septation mutants. GFP-bgs1⁺ bgs1 mutant cells were grown as in Fig. 2, shifted to 37°C for 3 hours (mid1-366) or 4 hours (cdc3-6, cdc11-119 and cdc14-118) or to 32°C for 5 hours (cdc15-140 and cdc16-116), and visualized for GFP and Calcofluor white staining. Cells representative of the different mutant phenotypes are shown.