Fig. 4. Bgs1p does not localize to the contractile ring during or after mitosis in the septation initiation network mutants cdc11-119 and cdc14-118. GFP-bgsl⁺ bgsl mutant cells were grown as in Fig. 2, shifted to 37°C and collected at different times (2.5, 3.5, 4.5 and 5.5 hours). Cells were ethanol fixed and analyzed for GFP and Calcofluor white/Hoechst staining. Cells representative of the mutant phenotypes at different times, including cells displaying a partial septum made before expressing the mutant phenotype, are shown.