Fig. 5. Bgs1p localizes to all around the cell and septum and to regions of altered cell wall deposition in the actin mutant cps8-188. GFP-bgs1⁺ bgs1 mutant cells were grown as in Fig. 2, shifted to 32°C for 5 hours or to 37°C for 3 or 6 hours, and examined for GFP and Calcofluor white staining. Cells representative of the different mutant phenotypes at each temperature and incubation time are shown.