Fig. 4. Analysis of sex bodies in wild type (a-d), Xist^trun (a'-d') and Xist^1lox (a''-d'') late pachytene and diplotene spermatocytes by DAPI staining and immunostaining for XY77 and M31 (DAPI, blue; SYCP3, red; green is XY77 in b,b',b'' and M31 in d,d',d''; sex body, arrowhead). In late pachytene wild type, Xist^trun and Xist^1lox spermatocytes the sex body protrudes from the edge of the nucleus (a,a',a'') and stains positively for XY77 (b,b',b''). Later, during diplotene, in wild type, Xist^trun and Xist^1lox spermatocytes the sex body becomes more densely staining for DAPI (c,c',c'') and in all three genotypes is M31-positive (d,d',d''). Bar, 5 µm.