Fig. 3. CK2 coprecipitates with chicken erythroid ankyrin. Whole cell lysate prepared from erythroid cells from 10-day-old chicken embryos (lane 1) or immunoprecipitates prepared from this lysate using ankyrin preimmune (lane 2) or immune sera (lane 3) were electrophoresed on a 12.5% SDS polyacrylamide gel and transferred to nitrocellulose. This filter was incubated with a rabbit antibody that recognizes the and ' catalytic subunits of CK2, followed by protein A conjugated to horseradish peroxidase. After washing, immunoreactive species were detected by enhanced chemiluminescence.