Fig. 10. The dominant-negative form of Rab5b inhibits the formation of LGP85-induced large vacuoles. COS cells were transiently co-transfected with LGP85 and FLAG-tagged wild-type Rab5b or FLAG-tagged Rab5bS34N, and after 36 hours post-transfection fixed and incubated with a rabbit polyclonal antibody to LGP85 (B,D) and a mouse monoclonal antibody to FLAG for labeling of wild-type and mutant Rab5b (A,C). Cells were visualized by confocal microscopy. Bars, 20 µm.