Fig. 2. The formation of large swollen vacuoles is specific to the overexpression of LGP85. COS cells were transiently transfected with LGP85 (A,B), LGP107 (C,D), or LGP96 (E,F), fixed and stained with each polyclonal antibody. Cells were visualized by immunofluorescence microscopy. Upper columns (A,C,E) show the LGP85, LGP107 and LGP96 staining, respectively, and the lower columns (B,D,F) show the corresponding phase-contrast micrographs. Bars, 20 µm.