Fig. 3. Cytochemical characterization of the LGP85-induced large swollen vacuoles. COS cells were transiently transfected with LGP85, and 36 hours post-transfection were fixed and double-labeled for LGP85 (left) and LAMP-2 (B, middle), LBPA (E, middle), EEA1 (H, middle), TfnR (K, middle), MPR300 (N, middle), and GM130 (Q, middle). Cells were visualized by immunofluorescence microscopy. The right columns show the merged images of LGP85 (red) and each marker (green). Bars, 20 µm.