Fig. 6. A subset of LGP85-induced large swollen vacuoles is accessible to transferrin, EGF and dextran added externally. COS cells transfected with LGP85 for 36 hours were incubated with Alexa594-transferrin (red in A-C), Texas Red-EGF (red in D-F), or Texas Red-dextran (red in G-I) as described in Materials and Methods. Cells were then fixed, stained with LGP85 (green in A, D and G), and visualized by confocal microscopy. The right columns (C,F,I) show the merged images of LGP85 (green) and each endocytic marker (red). Bars, 20 µm.