Fig. 7. Overexpression of LGP85 causes impairment of membrane traffic from early endosomes to late endosomes/lysosomes. COS cells transfected with LGP85 for 36 hours were incubated with Alexa594-transferrin (red in C and O), Texas Red-EGF (red in G and S), or Texas Red-dextran (red in K and W) as described in Materials and Methods. Cells were then fixed, stained with LGP85 (blue in left columns) and either EEA1 (green in B, F and J) or LAMP-2 (green in N, R and V), and visualized by confocal microscopy. The right columns (D, H, L, P, T and X) show the merged images of EEA1 (green in D, H and L) or LAMP-2 (green in P, T and X) and each endocytic marker (red). Bars, 20 µm.