Fig. 3. Fluorescence microscopy of methanol/acetone-fixed AK 13-1 cells detecting the chimera HK13-EGFP (A-H) together with microtubules using anti -tubulin antibodies in combination with Texas-Red-labelled secondary antibodies (A'-D') or actin filaments employing Texas-Red-coupled phalloidin (E'-H') prior to OV incubation (A,A',E,E'), during OV treatment (2 minutes in B,B',F,F' and 10 minutes in C,C',G,G'), and after a 50 minutes recovery period following a 10 minute OV-incubation (D,D',H,H'). OV concentration was 5 mM. Note the partial loss of microtubules and the increased peripheral localisation of actin during drug treatment, both of which are reversed after drug removal and do not reflect the dramatic changes of the CKF system. Bars, 10 µm.