Fig. 4. Fluorescence microscopy depicting the distribution of HK13-EGFP (A-K) together with plectin (A'-E'; monoclonal antibodies 10F6) or 14-3-3 (F'-K') in methanol/acetone-fixed AK13-1 cells in the absence of OV (A,A',F,F'), after a 2 minute OV treatment (5 mM; B,B',G,G'), a 10 minute OV treatment (5 mM; C,C',H,H'), after 2 or 3 hours of okadaic acid incubation (0.1 µg/ml; D,D',I,I') or during mitosis (E,E',K,K'). Note the increased co-distribution of plectin and CKs during OV treatment and mitosis and the lack of plectin staining of okadaic-induced granules, which is in contrast to 14-3-3 distribution exhibiting the reverse behavior. Bars, 10 µm.