Fig. 2. Absence of subnuclear organization of a mutant RUNX protein in fixed cells. SaOS-2 cells were transfected with either the wild-type EGFP-RUNX1, EGFP-RUNX2 or the mutant EGFP-RUNX2361 expression vectors. Both whole cell (WC) and nuclear-matrix—intermediate-filament (NMIF) preparations were performed as described in the Materials and Methods and show punctate foci for RUNX1 and RUNX2. The green fluorescence of EGFP was captured with a FITC filter (center images). Inserts show DAPI-stained nuclei (top left corners) and transmitted light photographs (lower right corners) of each cell. The scale bar equals 10 µm.