Fig. 5. RUNX1 and RUNX2 subnuclear foci are stationary in living cells within the nuclear space. SaOS-2 cells were transiently transfected with (A) EGFP; (B) EGFP-RUNX1; (C) EGFP-RUNX2 and (D) EGFP-RUNX2361 expression vectors. Time-lapse images were pictures captured at 0, 5, 10, 15 and 20 minutes (A) or 0, 5, 10, 20, and 30 minutes (B-D) using 100 milliseconds (A, B) or 200 milliseconds (C,D) exposure times. Arrowheads in B and C illustrate examples of stationary RUNX subnuclear domains. Scale bars equal 10 µm.