Fig. 6. RUNX1 and RUNX2 dynamically associate with subnuclear foci in living cells in a C-terminus-dependent mechanism. SaOS-2 cells were transiently transfected with (A) EGFP-RUNX1; (B) EGFP-RUNX2; (C) EGFP-RUNX2361 and (D) EGFP expression vectors. Pre-bleached images are shown. The circles represent the entire photobleached areas in which the recovery rates were calculated. The black boxes in A and B represent the area encompassing the foci within the photobleached area in which the recovery rates were determined. The images shown were captured before photobleaching and 1, 3, 5, 10 and 45 seconds after photobleaching. The white bars correspond to a scale of 10 microns. (E) Recovery curves of the proteins are shown as relative fluorescence intensity vs. time. From these curves the half-time of recovery and percent immobile fraction were calculated as described in the Materials and Methods. The line at a relative intensity of 1.0 represents the fluorescence intensity before bleaching.