Fig. 4. Localisation of insulin and Golgi apparatus in KHC^mut-expressing (a-c, g-i; arrow) and control (d-f, g-i) ß-cells. Cells were transfected with the KHC^mut-pAdTrack-CMV construct (A). Downstream of the multiple cloning site, the shuttle vector carries cDNA for EGFP driven by a distinct second CMV promoter. 48 hours after transfection the cells were fixed and probed with (a-f) a guinea-pig anti-insulin (1:500) or (g-i) a mouse monoclonal anti-TGN38 antibody (1:100) and then visualised with the appropriate Alexa 568 secondary antibodies. (a,d,g) Transmitted light images. (b,e,h) Alexa 568 fluorescence (568 nm excitation). (c,f,i) Intrinsic EGFP fluorescence (488 nm excitation). Bars, 2.5 µm (a-i).