Fig. 5. Transcriptional activity of Runx2/Cbfa1 is suppressed in the PDL-L2 cells. (A) PDL-L2 cells were cultured in the differentiation medium (D) with or without 250 ng/ml rhBMP-2 and gene expression was examined by RT-PCR. (B) Each cell line was transfected with p6OSE-2-Luc, and luciferase activity was determined. (C) Each cell line was transfected with p6OSE-2-Luc together with pcDNA3-Runx2/Osf2 or pcDNA3, and luciferase activity was determined. Each assay was carried out in a duplicate or triplicate. Each set of data is a representative of at least two independent experiments. See text for details.