Fig. 1. Expression of Dd-Wt, Ch-Ac and Ch-Sm in HS1 null cells. (A) Schematic representation of Dd-Wt, Ch-Sm and Ch-Ac heavy chains (modified figure reproduced with permission from Liu et al., 2000). (B) Filaments of purified Dd-Wt, Ch-Sm and Ch-Ac myosins (reproduced with permission from Liu et al., 2000). (C) Coomassie-blue SDS-PAGE of total cell extracts of AX3 cells and HS1 cells expressing Dd-Wt, Ch-Ac or Ch-Sm. (D) Westerns of SDS-PAGE of total cell extracts. AX3, HS1 and Dd-Wt cells were blotted with anti-DdMII, Ch-Ac cells were blotted with anti-Acanthamoeba myosin II and Ch-Sm cells were blotted with anti-smooth muscle myosin II. Anti-Acanthamoeba myosin and anti-smooth muscle myosin did not react with HS1 cells (not shown).