Fig. 2. Ch-Ac and Ch-Sm support growth and cytokinesis in suspension culture of transfected HS1 cells. (A) Growth rates of cells determined by cell number. (B) Representative cells stained with 4,6-diamidino-2-phenylindole after 3 days in suspension culture showing that transfected cells have many fewer nuclei than HS1 cells. (C) Quantification of number of nuclei per cell; the data are the mean of three experiments in each of which at least 100 cells were scored for each cell type.