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Fig. 4. Dynamics of localization of Ch-Ac and Ch-Sm to the cleavage furrow during cytokinesis. (A) Time course of localization of GFP-Ch-Ac to the cleavage furrow of a typical, live cell undergoing cytokinesis. Time is in seconds. (B) Gallery of images showing the localization by indirect immunofluorescence of Ch-Sm in cells at different stages of cytokinesis. The cells in B were aligned by matching them to similar images in the Ch-Ac sequence (A). Whereas Ch-Ac relocated symmetrically from the cortex to the cleavage furrow, the cleavage furrow in HS1 cells expressing Ch-Sm seemed to form at the site of the cortical patch of Ch-Sm, which remained on one side of the cleavage furrow until the furrow was highly constricted.