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Fig. 4. Lipid rafts influence the homoassociation of ErbB2. Lipid rafts were visualized with FITC-CTX-B, and ErbB2 was labeled with Cy3-4D5 and Cy5-4D5 antibodies in order to measure its homoassociation with the acceptor photobleaching FRET method. FRET efficiency was averaged in pixels with the same FITC-CTX fluorescence intensity, and the average FRET efficiency is plotted as a function of lipid raft labeling.